NAD+ 500mg
NAD+ (Nicotinamide Adenine Dinucleotide) is an endogenous coenzyme composed of two nucleotides — adenosine and nicotinamide riboside — joined through a pyrophosphate bridge. Supplied as a ≥99% HPLC-UV purity lyophilized powder, 500 mg labeled size, with identity confirmed by LC-MS ([M+H]⁺ observed at m/z 663.700). Lot-specific Certificate of Analysis from Freedom Diagnostics (USA). Research Use Only — not for human or veterinary use.
$62.00
| Quantity | Price | Discount |
|---|---|---|
| 5-10 | $59.52 | 4% |
| 11-15 | $57.04 | 8% |
| 16-20 | $54.56 | 12% |
| 21+ | $52.08 | 16% |
🧬 99%+ HPLC Purity
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NAD+ — full chemical name Nicotinamide Adenine Dinucleotide — is an endogenous pyridine dinucleotide coenzyme with an approximate molecular weight of 663.43 g/mol and CAS number 53-84-9 (free acid form). Structurally, NAD+ consists of two nucleotides joined through a pyrophosphate bridge: an adenosine 5′-monophosphate (AMP) moiety and a nicotinamide mononucleotide (NMN) moiety. This product is supplied as the oxidized form (NAD⁺); the reduced counterpart NADH is a chemically distinct compound not covered by this SKU.
The lot referenced here (NAD5-032-TPL) is supplied as a white lyophilized powder with a documented purity of 99.57% by HPLC with UV detection, and identity confirmed by LC-MS through convergent observation across two charge states — [M+H]⁺ at m/z 663.700 and [M+2H]²⁺ at m/z 332.600. Labeled vial size is 500 mg; net content per vial per the COA is 521.11 mg. Third-party characterization was performed by Freedom Diagnostics (USA). The product is intended exclusively for in-vitro laboratory research and analytical applications.
In the biochemistry research literature, NAD+ is described as a coenzyme that participates in oxidation-reduction reactions as an electron acceptor, alternating between its oxidized (NAD⁺) and reduced (NADH) forms.
| Compound Name | NAD+ |
|---|---|
| Full Chemical Name | Nicotinamide Adenine Dinucleotide (oxidized form) |
| Synonyms / Also Known As | NAD; NAD plus; β-NAD⁺; β-Nicotinamide adenine dinucleotide; oxidized nicotinamide adenine dinucleotide; DPN (diphosphopyridine nucleotide, historical name); coenzyme I (historical name) |
| CAS Number | 53-84-9 |
| Molecular Formula | C₂₁H₂₇N₇O₁₄P₂ (as the neutral zwitterion) |
| Molecular Weight | ~663.43 g/mol (free acid) |
| MS Confirmation on COA | [M+H]⁺ observed at m/z 663.700; [M+2H]²⁺ observed at m/z 332.600 (convergent identity confirmation across two charge states) |
| Structure Type | Dinucleotide (adenosine 5′-monophosphate + nicotinamide mononucleotide, joined via pyrophosphate bridge) |
| Component Nucleotides | Adenosine monophosphate (AMP) moiety + nicotinamide mononucleotide (NMN) moiety |
| Oxidation State | Oxidized form (NAD⁺); not the reduced form NADH |
| Compound Class | Endogenous pyridine dinucleotide coenzyme referenced in biochemistry research |
| Product Type | Coenzyme (endogenous metabolic cofactor) — not a peptide, not a designed small-molecule drug |
| Purity | 99.57% (HPLC-UV) — per COA lot NAD5-032-TPL |
| Identity Confirmation | Confirmed by LC-MS (per lot COA) |
| Appearance | White lyophilized powder |
| Solubility | Water-soluble |
| Hygroscopicity | NAD+ is hygroscopic and moisture-sensitive; strict moisture protection is essential |
| Available Sizes | 500mg |
| Net Content per Vial (COA) | 521.11 mg |
| Storage (lyophilized) | -20°C, sealed, protected from light and moisture; NAD+ can hydrolyze on prolonged storage at room temperature |
| Storage (reconstituted) | Aqueous solutions of NAD+ are less stable than the lyophilized form; use freshly prepared solutions where possible, aliquot and freeze -20°C for longer-term storage, avoid repeated freeze-thaw |
| Synthesis | Manufactured to research-grade specification |
| Testing Lab | Freedom Diagnostics, USA |
| Principal Chemist (on COA) | Alex Johnson |
| Test Methods | Purity by HPLC with UV detection; identity by LC-MS |
| Lot Number | NAD5-032-TPL |
| Accession Number (COA) | 2605280446 |
| Shipped From | USA |
| Certificate of Analysis | Lot-specific COA available |
The following describes investigative contexts in which this compound appears in the scientific literature. Provided for scientific background only; this does not describe any product use.
- Enzyme cofactor biochemistry — used as a reference cofactor in in-vitro enzyme-assay development for NAD⁺-dependent dehydrogenases and other oxidoreductase enzyme families.
- Analytical standard — used as a reference compound for HPLC-UV and LC-MS method development involving pyridine nucleotides.
- Redox biochemistry research — referenced as the oxidized member of the NAD⁺/NADH redox pair in in-vitro biochemistry studies.
- Dinucleotide structure/property studies — used as a reference dinucleotide in in-vitro biophysical characterization work.
For laboratory research handling only.
Reconstitution. Reconstitution solvent should be selected according to your experimental protocol and stability requirements. NAD+ is water-soluble; HPLC-grade ultrapure water or aqueous buffer at physiological pH is a common research-grade option. Aqueous solutions of NAD+ are less stable than the lyophilized form — the compound can slowly hydrolyze in solution, particularly at elevated temperature or extreme pH. Use freshly prepared solutions where possible. Add solvent slowly down the vial wall; allow to dissolve fully before use.
Storage — lyophilized. Store sealed at -20°C, protected from light and moisture. NAD+ is hygroscopic; strict moisture protection is essential. Warm the sealed vial to room temperature before opening to minimize moisture condensation on the powder.
Storage — reconstituted. Use freshly prepared solutions where possible, or aliquot and freeze at -20°C for longer-term storage. Avoid repeated freeze-thaw cycles. Determine working concentrations using your own validated laboratory methods.
NAD+ 500mg — Frequently Asked Questions
What is NAD+?
NAD+ (Nicotinamide Adenine Dinucleotide) is an endogenous pyridine dinucleotide coenzyme composed of two nucleotides — an adenosine 5'-monophosphate moiety and a nicotinamide mononucleotide moiety — joined through a pyrophosphate bridge. It is supplied as a white lyophilized powder for laboratory research use only.
Is NAD+ a peptide?
No. NAD+ is a coenzyme — specifically a pyridine dinucleotide — not a peptide. It is composed of two nucleotides joined by a pyrophosphate bridge, and does not contain amino acids or peptide bonds.
What is the difference between NAD+ and NADH?
NAD+ is the oxidized form of the coenzyme; NADH is the reduced form. This product is supplied as the oxidized form (NAD+) only. NADH is a chemically distinct compound and is not covered by this SKU.
What is the molecular weight and CAS number of NAD+?
NAD+ has a molecular formula of C21H27N7O14P2 and an approximate molecular weight of 663.43 g/mol. The CAS number for the free acid form is 53-84-9. The lot LC-MS confirmation on this product observes NAD+ at [M+H]+ 663.700 and [M+2H]2+ 332.600 across two charge states.
What sizes and purity are available?
NAD+ is available in multiple vial sizes; this SKU is 500 mg labeled with 521.11 mg net content per the lot COA. Purity is 99.57 percent by HPLC with UV detection, with identity confirmed by LC-MS. A lot-specific COA from Freedom Diagnostics (USA) is available.
How should NAD+ be stored?
Store lyophilized material sealed at -20C, protected from light and moisture. NAD+ is hygroscopic; strict moisture protection is essential. Warm the sealed vial to room temperature before opening to minimize moisture condensation on the powder. Aqueous solutions are less stable than the lyophilized form; use freshly prepared solutions where possible, or aliquot and freeze at -20C, avoiding repeated freeze-thaw cycles.
Is a Certificate of Analysis (COA) available?
Yes. Each lot has a Certificate of Analysis from an independent third-party laboratory (Freedom Diagnostics, USA) documenting HPLC purity and LC-MS identity confirmation, tied to the vial lot number. The reference lot for this product line is NAD5-032-TPL.
Do you provide dosing, route-of-administration, or usage instructions for NAD+?
No. All products are supplied strictly for laboratory research use only. We do not provide dosing, administration, or route-of-administration guidance of any kind — this includes oral, sublingual, intravenous, subcutaneous, and any other route. We do not provide protocols, calculators, or usage guidance. Researchers determine experimental parameters according to their own validated protocols and applicable regulations.
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